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Purospher® - High-purity HPLC columns

Purospher® STAR Si and Purospher® STAR NH2

Purospher® STAR RP-8 endcapped

Purospher® STAR HPLC columns based on highest purity silica are also available for normal phase separations.

Purospher® STAR Si (Silica) offers highest separation efficiency for normal-phase chromatography of low molecular weight compounds soluble in organic solvents.

Purospher® STAR NH2 (Amino) is primarily designed for carbohydrate analysis with a typical mobile phase consisting of acetonitrile and water. Additionally, Purospher® STAR NH2 can also be used in the normal-phase retention mode.

 

Key performance benefits:
  • Very high separation efficiency as measured by the plate count
  • Absence of metal impurities, thus giving consistently symmetrical peaks
  • Extended column lifetime
Specification of Purospher® STAR Si and Purospher® STAR NH2
 Purospher® STAR SiPurospher® STAR NH2
Sorbent characteristics High purity silica gel With NH2 (amino) modification
Metal content Na, Ca, Mg, Al: 1 ppm; Fe: 3 ppm Na, Ca, Mg, Al: 1 ppm; Fe: 3 ppm
Particle shape spherical spherical
Particle size 5 μm 5 μm
Pore size 120 A (12 nm) 120 A (12 nm)
Pore volume 1.1 mL/g 1.1 mL/g
Spec. surface area 330 m²/g 330 m²/g
Carbon load   3.5 %
Coverage of the surface 3 µmol/m² 3 µmol/m²
pH range pH 2-7.5 pH 2-7.5
Efficiency > 50.000 N/m > 50.000 N/m
Shipping eluent n-Heptane n-Heptane
Separation of Carbohydrates on Purospher® STAR NH2
Column LiChroCART® 250-4 Purospher® STAR NH2, 5 µm Separation of Carbohydrates on Purospher® STAR NH2
Eluent Acetonitrile/Water (75:25)
Flow rate   1.0 mL/min
Detection RI
Temperature 30°C
Inj. volume 10 µL
Sample 1. Xylose
2. Fructose
3. Glucose
4. Saccharose
5. Maltose
6. Lactose
Separation of Anisoles on Purospher® STAR Silica
Column LiChroCART® 125-4 Purospher® STAR Si, 5 µm Separation of Anisoles on Purospher® STAR Silica
Eluent n-Heptane/Dioxane (95/5, v/v)
Flow rate   2.0 mL/min
Detection UV 254 nm fast response
Temperature RT
Inj. volume 5 µL
Sample

1. Anisole
2. 3-Nitroanisole
3. 4-Nitroanisole
4. 2-Nitroanisole

 

© Merck KGaA, Darmstadt, Alemania, 2012


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